Abadia-Chanona QY, Avila-Poveda OH, Arellano-Martinez M, Ceballos-Vazquez BP, Benitez-Villalobos F, Parker GA, Rodriguez-Dominguez G, Garcia-Ibañez S (2018) Reproductive traits and relative gonad expenditure of the sexes of the free spawning Chiton articulatus (Mollusca: Polyplacophora). Invertebrate Reproduction and Development 62(4): 268-289.
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After 3 days of recovery from the microelectrode implantation, animals were recorded in free-movement conditions for 60 min/day on days 19, 20, 21, 25, and 32 (5 days) after the first spontaneous seizure. The recordings were performed in a polygraph (Model 7D, Grass Technologies, RI, USA) at a bandwidth of 0.1 to 5 kHz, sampling at 5 kHz per channel (3 channels) with 12-bit precision using an iMac A1048 (Apple, USA) and MP150 (BIOPAC Systems, CA, USA) as an analog to digital converter, and AcqKnowledge Data Acquisition software 4.0 as the user interface (BIOPAC Systems, USA).
Ranges for each variable were as follows: peak-to-peak amplitude from 1 to 150 μV; power frequency from 250 to 600 Hz; duration from 1 to 500 ms; and power from 1 to 150 \(\frac\mu V^2Hz .\) Finally, the likelihood of each fast ripple parameter (P(Xθ)) was modeled as a T distribution with the mean and standard deviation observed in each region of the trisynaptic circuit. Degrees of freedom (ν) of the T distribution followed an a priori exponential distribution with 30 mean, which favors the regions with normally distributed data over the tails. All the main procedures followed the BEST method [43].
Consumption of equine meat is still reduced compared to other meats such as pork, chicken and beef (LOMBARDI-BOCCIA et al., 2005). However, equine meat presents desirable characteristics for consumers as they are free of growth promoters and has excellent nutritional properties (equine meat is iron-rich, low fat and an important source of unsaturated fatty acids and omega 3 fatty acids) (LOMBARDI-BOCCIA et al., 2005; TATEO et al., 2008; LORENZO et al., 2010; LORENZO et al., 2014).
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